Calcium. 2-µL or 4-µL samples were pipetted onto a 64-well SpectraDrop slide in quadruplicate, following the plate map in Figure 2. o For example: Ex: 485/Em 525/Cutoff 515 4. However, no piece of equipment is immune to breaking down. Ease of use. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. SpectraMax M5. 3) At the bottom of the strip, click on "Show. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. ü; ü. FlexStation® 3 Multi-Mode Microplate Reader. SpectraMax i3x Multi-Mode Detection Platform. SpectraMaxParadigmMulti-ModeDetectionPlatformUserGuide 4 5014038F Chapter5:ReadModesandReadTypes 71 SupportedReadTypes 71 AbsorbanceReadMode 74 FluorescenceIntensityReadMode 79the SpectraMax Paradigm Multi-Mode Detection Platform User Guide. . 2. ClonePix 2 Mammalian Colony Picker; QPix Microbial Colony Pickers; CloneSelect Imager; DispenCell Single-cell Dispenser; 3D. Examples: “Press carefully to engage,” “ Do not. Enzyme Activation Analysis;. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. The SpectraMax i3x is a multi-mode microplate reader that measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. SpectraMaxi3Multi. It allows you to explore cellular pathways and protein activation and expression in one system. 6% for the on-board optics (monochromators). Research Article AntioxidantActivity,PolyphenolicContent,andFT-NIR AnalysisofDifferentAspiliaafricanaMedicinalPlantTissues DenisOkello ,1,2,3YuseongChung,1HyoseonKim. Monday to Friday 8am - 5pm GMT. Unplug the power cable from the back of the instrument. One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. Results were plotted as ratio of green/red vs. The SpectraMax i2X is used for cell migration, cell. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. SpectraMax i3x Multi-Mode Detection Platform. The plate was shaken and incubated in the dark for 10 minutes. For researchers working in GLP or GMP laboratories, achieve full GxP compliance with our turnkey solution:. Supported Windows Operating System: See release notes. TP medium was used as a blank and its fluorescence intensity was subtracted from that of mCherry or from chlorophyll auto-fluorescence intensity. Get a front row view of your cells. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. ZERO BIAS - scores, article reviews, protocol conditions and moreStructured Review. . . SpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. Multimode Reader Comparison Chart. For each kit, the reader was able to detect the lowest dilution as positive. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. This process should take about 3 - 5 minutes. 0. 2. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Catalog excerpts. Supplier: Molecular Devices. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. Figure 3. Expand your lab’s capabilities to include flash applications such as dual luciferase and ATP assays with the SpectraMax® i3x injector module with SmartInject® Technology ensuring equal mixing across the plate for high-precision experiments. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. Note: Additional readers with similar performance are listed. 315 can't find zero order. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Find regional support. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Remove the transport bracket and store the retaining screws in the bag that held the 3. Multimode Reader Comparison Chart. The SpectraMax DuoLuc Reporter Assay has been performance optimized on the SpectraMax i3x reader with Injector Cartridge. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. The solutions were thoroughly mixed and then incubated in the dark at 20°C for 2 h. The user guide also covers the features, specifications, and software of the reader, as well as the installation and. Check software compatibility with Windows 10 and locate your software installer. com. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). SpectraMax iD3 and iD5 Register or Sign In. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. \r Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48 AM The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. . 99 to $1999. The calculated LOQ values were 0. 100 fmol 1536-well (8 µL) 0. Ribonuclease (RNase) R digestion assay For identifying the stabilization of circVRK1, 2 μg isolated RNA from HBMECs was digested by RNase R (3 U/μg; RNR07250, Geneseed, Guangzhou,. Homogeneous and eterogeneous microplate assays can be performed in one flexible system. Monday to Friday 7am - 5pm Pacific Time. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. to View Figures. using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. 58% for the detection module and 2. The dual auto injector module. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part. Current methods available to rapidly detect these can be resource intensive and thus sub-optimal. . The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. 2. . Engineered for high performance, the auto dual injector cartridge includes fail safe features. . 0 mm hex key to remove the six blue retaining screws that secure the transport bracket (B) to the instrument. The dynamic range of the assay is about 3. The SpectraMax M4 reader had identical performance specifications to the SpectraMaxLuminescence was read on the SpectraMax i3x reader and the SpectraMax L reader. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. 304 signal level saturation. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. 세포신호전달은 세포가 환경에 반응하고 다른 세포와 소통할 수 있게 합니다…. com. • SpectraMax i3x • Onboard luminescence • Detection cartridge (LUM 96) • SpectraMax iD5 • SpectraMax L • SpectraMax M5e • SpectraMax Mini. . Molecular Devices推出的SpectraMax® i3x 多功能酶标仪除了具有全波长的光吸收、荧光、化学发光和FRET检测功能以外，还兼容了客户端模块化升级功能，用户可以根据需要任何时间随意升级至荧光偏振FP、HTRF、AlphaScreen、Western Blot、上转发光检测、细胞成像和带有注射器. Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. 99. Find regional support. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and. Simplified workflow: Add cellular imaging assays in a small footprint. Product Details. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. Video: SpectraMax i3x Overview; Brochure: SpectraMax i3x Multi-Mode Detection PlatformVersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Seller Details. The corrected absorbance is within 5% of the. 1321N1, a human astrocytoma cell line. The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. At each time point, the samples were shaken and fluorescence was measured for SEP (488-nm excitation and 530-nm emission) and. Sufficient for 1000 reactions. Table 1. All the readers were operated using SoftMax® Pro Software, which also has built-in data analysis and curve-fitting for automatic calculation of EC 50. SpectraMax® Microplate Readers and SoftMax® Pro Software. More Product Information. 性能特点MD SpectraMax i3X 多功能荧光检测仪为采用光谱融合照明设计、测试功能模块化卡盒设计，具有细胞成像分析单元。. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. . Download PDF. 5mM EDTA, 1% NP40,Explore a wealth of applications with the future-ready SpectraMax i3x. Explore a wealth of applications with the future-ready SpectraMax i3x. Use the 3. RIDASCREEN Gliadin competitive ELISA. Find regional support. Dilution series of HeLa cells transfected with firefly (red plot) and Renilla (blue plot) luciferases. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Download SoftMax® Pro 7. Align the two slots on the bottle holder with the two pegs on the rail. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. . Products : SpectraMax i3x reader, SoftMax Pro GxP Software, SpectraMax i3, SoftMax Pro Software, SpectraMax MiniMax cytometer When I attempt a read with my SpectraMax i3 or i3x reader using the injector cartridge, why do I see a plate height error, even though I have entered the correct plate height?. Table 1. SpectraMax i3xは、吸光・蛍光・発光スペクトル測定のためのマルチモードマイクロプレートリーダーです。. Description 1. Spectramax I3x, supplied by Danaher Inc, used in various techniques. In the second assay, HepG2 cells were plated into a 96-well clear-bottom, blackwalled plate at 10,000 cells/well and incubated overnight at 37°C. Request a Quote for This Setup. For more information on the SpectraMax i3x Multi-Mode Microplate Reader System, please click here. Description 1. We would like to show you a description here but the site won’t allow us. Italic and boldface type are used for emphasis. These robust readers have been placed in labs from Antarctica to the. The MiniMax cytometer with SoftMax® Pro Software can be used to image and analyze a variety of cells. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Bioz Stars score: 98/100, based on 1 PubMed citations. Eight different automated tests are provided: • Absorbance accuracy (linearity) • Precision (reproducibility) • Stray light • Wavelength accuracy • Wavelength repeatability SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. SpectraMax ® i3/i3x Multi-Mode Microplate Reader; SpectraMax Plus 384 Absorbance Microplate Reader; Methods. 2. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. Wait 20 seconds. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. Description 1. SpectraMax i3x Multi-Mode Microplate Reader. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. NORTH AMERICA. Microplate Reader Shelf. Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). to View Figures. Shaking and incubations were performed using an on-deck orbital shaker. After 2 days of incubation, LUC and REN activities were measured using a SpectraMax ® i3x Multi-Mode detection platform (Molecular Devices, USA) with a Dual-Luciferase Reporter Assay Kit (Promega. Shake intensity. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 58% for the detection module and 2. The ELISA plates were read at an absorbance of 450 nm on a SpectraMax i3x Plate Reader using Softmax Pro 7. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellEvery SpectraMax ® Paradigm Detection Cartridge starts with an application optimized excitation energy source either in the form of a long life, high powered LED, xenon flash lamp, or high powered laser. Figure 1. Microplate Spectrophotometers. 과 같은 Parameter를 검사하여 평가할 수 있습니다. Settings not required by a reader are indicated by ‘---’. Open the catalog to page 1. SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax. The StemoniX microBrain 3D platform is a homogenous 3D neural spheroid system in 96 and 384-well format. Summary of Contents for Molecular Devices SpectraMax M2. 003 fmol 0. Finally, their fluorescence was measured with 365-nm excitation and 450-nm emission filters using a microplate fluorometer (SpectraMax i3x, Molecular Devices, San Jose, CA, USA) (Saiya-Cork et al. . The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringfluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiterA microplate reader that can detect two or more applications is considered a multi-mode plate reader. Danaher Inc spectramax i3x. The plate was shaken and incubated in the dark for 10 minutes. Fluorescence measurements of the soil solutions and enzyme activities were made using a microplate reader (SpectraMax i3x, Molecular Devices, USA). Data analysis. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes With these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. pathways and protein activation and expression in one system. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . We would like to show you a description here but the site won’t allow us. Note: Additional readers with similar performance are listed. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. 312 gain calibration failed. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. It allows you to explore cellular pathways and protein activation and expression in one system. Facilities. Spectranet customer portal Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Download PDF. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. SpectraMax® i3x Multi-Mode Detection Platform; SpectraMax® i3 Multi-Mode Detection Platform; SpectraMax® ID3 Multimode Microplate reader; SpectraMax® ID5 Multimode Microplate reader; SpectraMax® Paradigm® Multi-Mode Microplate Reader; SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. were measured at OD405 using a SpectraMax i3x multimode detection system (Molecular Devices, San Jose, CA, USA), and the melanin concentration was determined using the standard curve of melanin solutions with known concentrations. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Average 98 stars, based on 1 article reviews. 001 0. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. . Description 1. #0200-7009) Contact our support team with your questions regarding Molecular Devices products and applications. Explore PDF for more details. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. . . The Curcumin binding to p53Y220C was also measured using the emission maxima of Curcumin at 495 nm after. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Viewing this answer requires an authorized account. The SpectraMax i3x reader provides theSoftMaxProSoftwareFormulaReferenceGuide 8 5049093A SummaryFormulas Summariesconsistoffourparts: ASummaryname(whichisusedtorefertotheSummaryinotherformulas). INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideProducts : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. Introduction Inflammation, the body’s reaction to infection, irritation, or other injury, is accompanied by increased endothelial chemokine. Reader spectramax microplate id3 molecular devices multi mode boundaries functionality instrumentSpectramax i3x multi-mode microplate reader with minimax imaging Molecular devices spectramax i3x 使用される 販売のために 価格. Microplate readers, multi-mode, SpectraMax® i3x. 012 fmol 0. 5577 Web: Email: info@moldev. The NET-specificity of the detected signal was determined by incubation with DNase I (50 U/mL, Qiagen, Germany). Spectramax I3x, supplied by Danaher Inc, used in various techniques. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. . Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Serial number 33 900 01-3387. Example: [Drawer]. 303 unable to cal dark current. The SpectraMax i3x is a multi-mode microplate reader that measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. 5 decades, with sensitive detection of cells. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. Flash-type luminescence with excellent sensitivity Expression measurement in as few as ten cells per well Improved accuracy of results by. Absorbance Fluorescence (top/bottom read) Luminescence (top read) Time-Resolved Fluorescence (top read). . HTRF IP standard curve. Immunofluorescence of In Vitro LPS-Stimulated Neutrophils. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application. SpectraMax® ABS and ABS Plus reader support; ü. . . detection cartridge LUM96: 59. Tristar 5 Multimode Microplate Reader. 0´ 104 1. Published Dec 16, 2020 | Updated Dec 16, 2020. . A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. The dual auto injector module. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. ZERO BIAS - scores, article reviews, protocol conditions and more. Molecular Devices ScanLater Western Blot Detection Platform. Both readers demonstrated their ability to detect the HTRF signal from the terbium donor and red acceptor combination, and they could be used for saturation studies using the Tag-lite technology. 030 fmol. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. Each heating element is independently. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Spectranet customer portalThe SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. SpectraMax i3x, onboard luminescence 121. Nuclear Receptor. SpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. SpectraMax i3x and Paradigm readers require the FP-FLUO. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataSpectraMax i3x Multi-Mode Microplate Reader. . 313 reference gain check fail. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Results for SpectraMax i3x reader with NanoBRET Detection Module (blue) and on-board luminescence detection mode (orange). The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. 660-665 Eskdale Road. Time-. Negative samples contained only MycoAlert Assay Buffer. Published Dec 16, 2020 | Updated Oct 10, 2023. SpectraMax Microplate Readers. i3x. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. TaqMan SNP. 1. 1. 635. Background Parallax,Columns 2. 1 1 10 100 e e e e e e eSpectraMax Spectrophotometers for UV-Vis Absorbance Detection. ImageXpress Confocal HT. 2 x 10-14. The. Core features include biological and fume hoods, a cold room and multiple freezers, and access to institutional space for radiolabeling and radiation monitoring. 0´ 104 6. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析We would like to show you a description here but the site won’t allow us. 1. 2. i3. The CellTiter-Glo assay from Promega uses luciferase. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. Find regional support. Setting iD5 i3x M5 Paradigm Measurement mode Fluorescence Polarization Fluorescence Polarization Fluorescence Polarization Fluorescence Polarization Excitation 485 nm monochromator 485 nm 485 nm 485 nmPage 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. 305 reference level saturation. It allows you to explore cellular pathways and protein activation and expression in one system. 100 fmol 384-well (75 µL) 0. ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . The SpectraMax i3x Multi-Mode Microplate Reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. Molecular Devices are known for both the reliability and the wide uses of their products. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Order Now. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. If no sound is heard during initialization, contact technical support. 020 fmol 0. 7. As. 0. Bioz Stars score: 98/100, based on 1 PubMed citations. Videos & Webinars. Figure 4. The SpectraMax i3x’s user-upgradeable application modules will future-proof your research to include most assay capabilities of the SpectraMax iD3 and iD5 readers, as well as additional capabilities such as imaging. Spectramax I3x, supplied by Danaher Inc, used in various techniques. 01 0. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. SpectraMaxMiniMaxImagingCytometerInstallationGuide 10 5024111D AttachingtheCytometertotheMicroplateReader Prepareaworkareathatisapproximately130 cm(51. 2in. . Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. In order to calculate a humification index (HIX), spectra were obtained with an excitation wavelength of 254 nm over the emissions waveband of 300–480 nm at increments of 5 nm (Ohno 2002. The 4x washing steps were also performed on deck using the Span-8 aspirate and dispense for the first three washes.